Haematology

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Notes

Serum folate assay is now performed on the Abbott Alinity platform as of 10th September 2024.

Patient must be fasting for a minimum of 12hrs

Queries to Haematinics Laboratory on 01-4162012

Haematinics Demand Management Guidelines:

1. Patients on parenteral vitamin B12 or oral folic acid replacement therapy should not have further vitamin B12 or folic acid measurements performed unless blood counts or neurological symptoms fail to improve.

2. Repeating normal vitamin B12 levels is rarely indicated within 6 months of testing.

3. Repeating normal folate levels is rarely indicated within 3 months of testing.

4. Clinical details are required for all B12 and folate requests. Compliance will be monitored by periodical auditing.

Limitations of the assay:

• Haemolysed serum samples are unacceptable for analysis and give falsely raised folate results. 
• Specimens from patients who have received preparations of mouse monoclonal antibodies for diagnosis or therapy may contain human anti-mouse antibodies (HAMA). Such specimens may show either falsely elevated or depressed values when tested with assay kits such as Alinity i Folate that employ mouse monoclonal antibodies. Additional information may be required for diagnosis.
• Heterophilic antibodies in human serum can react with reagent immunoglobulins, interfering with in vitro immunoassays. Patients routinely exposed to animals or to animal serum products can be prone to this interference, and anomalous values may be observed. Additional information may be required for diagnosis.
• Samples to be tested for folate should be protected from light. Light accelerates the degradation of folate.
• Serum and plasma specimens from patients with renal impairment or failure (including dialysis patients) may exhibit varying degrees of falsely depressed folate values. Therefore, additional information may be required for diagnosis.
• Methotrexate, aminopterin, and folinic acid (Leucovorin) are chemotherapeutic agents whose molecular structures are similar to folate. These agents cross react with folate binding protein in folate assays.

Related links

Reference range

Please note new reference range from 10th September 2024.

3.8 -18.2 ug/l

Last updated

Mon, 09 Sep 2024 16:03:14 IST