IMRL
| Full details for "Mycobacteria - Antibiotic Susceptibility Testing". | |
|---|---|
Name |
Mycobacteria - Antibiotic Susceptibility Testing |
Alternative name/Profile |
tubercle bacillus / TB / Mycobacteria tuberculosis / M. tuberculosis complex / MTC / M. africanum / M. bovis / M. bovis BCG / M. caprae / M. microti / M. canetti / tuberculin skin test / Bacillus Calmette-Guérin ( BCG ) vaccine / Mantoux test / acid fast bacillus / AFB / acid-fast stain / Ziehl-Neelsen stain / ZN stain / Kinyoun stain / fluorescence microscopy / auramine-rhodamine stain / Auramine O / Rhodamine B / hazard group 3 / category 3 pathogen / streptomycin / isoniazid / rifampicin / ethambutol / pyrazinamide / mycobacterium / M. chimaera |
Department |
IMRL |
Investigation |
|
Specimen type |
MTC Culture / Referred culture |
Sample type |
|
Sample container & volume |
Sterile microtube or LJ slope packed in accordance with ADR regulations as outlined in the IMRL user manual |
Frequency of analysis |
Daily |
Turnaround time |
14 - 21 days |
Availability |
Monday - Friday |
Notes |
Cultures that are positive for acid fast bacilli that originate from the Microbiology departments of client hospitals will be accepted for processing by the Irish Mycobacteria Reference Laboratory (IMRL). Laboratory numbers should be assigned to the cultures before they are forwarded to the IMRL and this number will be quoted on all future correspondence from the IMRL. Changes in the ADR regulations 2007 allow for the transport of MTC by road, under the category UN3373, “Biological Substance , Category B” . Packing instructions P650 apply. The turnaround time for MTC antibiotic susceptibility testing is greatly affected by the concentration of organisms in the culture received. The following first line anti-TB drugs are tested routinely at the IMRL: Streptomycin (1.0 μg/ml, 4.0 μg/ml); Isoniazid (0.1 μg/ml, 0.4 μg/ml); Rifampicin (1.0 μg/ml); Ethambutol (5.0 μg/ml); Pyrazinamide (100 μg/ml). Susceptibility testing to the following second line anti TB agents is available on request: Fluoroquinolones: Moxifloxacin (0.25μg/ml, 2.0 μg/ml); Ofloxacin (2.0 μg/ml) Aminoglycosides: Kanamycin (2.5 μg/ml), Amikacin (1.0 μg/ml), Capreomycin (2.5 μg/ml) When a specimen is procured it must be sent to the laboratory immediately. Batching of specimens is not recommended. Should a delay be unavoidable (e.g. weekends) specimens should be refrigerated until transported |
Related links |
|
Reference range |
|
Last updated |
Fri, 12 Jan 2024 09:53:30 GMT |