Full details for "Molecular Identification of Mycobacteria". | |
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Name |
Molecular Identification of Mycobacteria |
Alternative name/Profile |
tubercle bacillus / TB / Mycobacteria tuberculosis / M. tuberculosis complex / MTC / M. africanum / M. bovis / M. bovis BCG / M. caprae / M. microti / M. canetti / M.chimaera / Non-Tuberculous Mycobacteria / NTM / M. avium-intracellulare complex ( MAC ) / M. avium / M. chelonae / M. abscessus / M. fortuitum / M.gordonae / M. intracellulare / M scrofulaceum / M interjectum / M. kansasii / M malmoense / M peregrinum / M. marinum / M. ulcerans / M. xenopi / M. simiae / M. mucogenicum / M. goodii / M. cellatum / M. smegmatis / M. genavense / M. lentiflavum / M. heckeshornense / M. szulgai / M. intermedium / M. phlei / M. hemophilum / M. gastri / M. asiaticum / M. shimoidei / tuberculin skin test / Bacillus Calmette-Guérin ( BCG ) vaccine / Mantoux test / acid fast bacillus / AFB / hazard group 3 / category 3 pathogen / Polymerase Chain Reaction / PCR / nucleic acid hybridisation / nucleic acid amplification / reverse hybridisation / Hybridization Protection Assay ( HPA ) technology / DNA strip technology / line probe assay / resistance genes / rifampicin / isoniazid / Ziehl-Neelsen stain / ZN stain / Kinyoun stain / fluorescence microscopy / auramine-rhodamine stain / Auramine O / Rhodamine B / hazard group 3 / category 3 pathogen / Mycobacterium / M. chimaera |
Department |
IMRL |
Investigation |
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Specimen type |
MTC Culture / Referred culture |
Sample type |
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Sample container & volume |
Sterile microtube or LJ slope packed in accordance with ADR regulations as outlined in the IMRL User Manual |
Frequency of analysis |
Daily for MTC; Weekly for NTM |
Turnaround time |
1 - 7 days |
Availability |
Monday - Friday |
Notes |
All cultures that are positive by ZN and are morphologically typical Nontuberculous mycobacteria (NTM) may be processed using a GenoType Mycobacterium CM (for common mycobacteria) and/or GenoType Mycobacterium AS (for additional species), and/or GenoType NTM-DR (for the confirmation of M. chimaera) (Hain Lifescience GmbH, Nehren, Germany). M. tuberculosis Complex isolates may be tested for mutations in genes associated with drug resistance (GenoType MTBDRplus and MTBDRsl assays). GenoType assays are commercial assays that are based on the reverse hybridisation of PCR products to their complementary probes which are coated on nitrocellulose strips (DNA•STRIP®s). Additional gene sequencing methods (heat-shock protein, 16S and inter-spacer regions of the mycobacterial genome) may also be used when first line testing with commercial identification assays fail to identify the mycobacteria. Results should be interpreted in conjunction with other laboratory and clinical data available to the clinician. The presence of contaminating organisms, (e.g. GNB, GPCs) greatly affects the turnaround times of molecular techniques. When a specimen is procured it must be sent to the laboratory immediately. Batching of specimens is not recommended. Should a delay be unavoidable (e.g. weekends) specimens should be refrigerated until transported. |
Related links |
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Reference range |
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Last updated |
Fri, 12 Jan 2024 09:53:30 GMT |