IMRL
| Full details for "Molecular Identification of Mycobacteria". | |
|---|---|
Name |
Molecular Identification of Mycobacteria |
Alternative name/Profile |
tubercle bacillus / TB / Mycobacteria tuberculosis / M. tuberculosis complex / MTC / M. africanum / M. bovis / M. bovis BCG / M. caprae / M. microti / M. canetti / M.chimaera / Non-Tuberculous Mycobacteria / NTM / M. avium-intracellulare complex ( MAC ) / M. avium / M. chelonae / M. abscessus / M. fortuitum / M.gordonae / M. intracellulare / M scrofulaceum / M interjectum / M. kansasii / M malmoense / M peregrinum / M. marinum / M. ulcerans / M. xenopi / M. simiae / M. mucogenicum / M. goodii / M. cellatum / M. smegmatis / M. genavense / M. lentiflavum / M. heckeshornense / M. szulgai / M. intermedium / M. phlei / M. hemophilum / M. gastri / M. asiaticum / M. shimoidei / tuberculin skin test / Bacillus Calmette-Guérin ( BCG ) vaccine / Mantoux test / acid fast bacillus / AFB / hazard group 3 / category 3 pathogen / Polymerase Chain Reaction / PCR / nucleic acid hybridisation / nucleic acid amplification / reverse hybridisation / Hybridization Protection Assay ( HPA ) technology / DNA strip technology / line probe assay / resistance genes / rifampicin / isoniazid / Ziehl-Neelsen stain / ZN stain / Kinyoun stain / fluorescence microscopy / auramine-rhodamine stain / Auramine O / Rhodamine B / hazard group 3 / category 3 pathogen / Mycobacterium / M. chimaera |
Department |
IMRL |
Investigation |
|
Specimen type |
MTC Culture / Referred culture |
Sample type |
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Sample container & volume |
Sterile microtube or LJ slope packed in accordance with ADR regulations as outlined in the IMRL User Manual |
Frequency of analysis |
Daily for MTC; Weekly for NTM |
Turnaround time |
1 - 7 days |
Availability |
Monday - Friday |
Notes |
Once a sample has been cultured and has grown mycobacteria, the IMRL can use a number of molecular methods to identify the organism: ZN staining – to determine whether the organism is ‘typical’ (looks like TB), or ‘atypical’ (looks like non-tuberculous mycobacteria, e.g. M. avium or M. chelonae (~170 NTMs have been identified to date). We use whole genome sequencing to identify the members of the Mycobacterium tuberculosis Complex, and we use a mixture of line probe DNA hybridisation assays and Sanger sequencing to identify all other mycobacteria. Please refer to IMRL User Manual for more information. Results should be interpreted in conjunction with other laboratory and clinical data available to the clinician. The presence of contaminating organisms, (e.g. GNB, GPCs) greatly affects the turnaround times of molecular techniques. When a specimen is procured it must be sent to the laboratory immediately. Batching of specimens is not recommended. Should a delay be unavoidable (e.g. weekends) specimens should be refrigerated until transported. |
Related links |
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Reference range |
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Last updated |
Thu, 23 Jan 2025 12:39:04 GMT |