Immunology

Full details for "Immunofixation (Serum) ".
Name	Immunofixation (Serum)
Alternative name/Profile	IF, Electrophoresis, ELEC, GAM, Immunoglobulins, Multiple Myeloma, Lymphoproliferative Disease, Primary Systemic Amyloidosis, Light-chain Deposition Disease and Monoclonal Gammopathy of Unknown Significance (MGUS), FKFL
Department	Immunology
Investigation	Immunofixation (Sebia Hydrasys Focusing)
Specimen type	Clotted blood
Sample type	Serum
Sample container & volume	5ml Red.
Frequency of analysis	Daily
Turnaround time	7 days
Availability
Notes	Useful for: Idetification of the immunoglobulin heavy and/or light chain present in monoclonal gammopathies. Monitoring monoclonal gammopathies. Clinical Information: A monolonal expansion of plasma cells (mature B-lymphocytes) is seen in patients with a Monoclonal Gammopathy. Monoclonal gammopathies include Multiple Myeloma, Lymphoproliferative Disease, Primary Systemic Amyloidosis, Light-chain Deposition Disease and Monoclonal Gammopathy of Unknown Significance (MGUS). Serum proteins may be divided into six fractions using electrophoresis; the albumin, alpha-1, alpha-2, beta-1, beta-2 and gamma regions. A monoclonal expansion of plasma cells results in a monoconal production of immunoglobulin which can be detected on electrophoresis. Frequently monoclonal free light chains cannot be detected or quantified on electrophoresis. All samples that have abnormal immunoglobulin levels and/or an abnormality on electrophoreis will have immunofixation performed to characterise the monoclonal protein present. Please Note: Occasionally monoclonal proteins detected on immunofixation cannot be detected on electrophoresis. On these occasions it is not possible to quantify the paraprotein level. Where External Institiutions cannot guarantee immediate delivery, samples must be centrifuged, separated and stored at 4^oC. Samples can then be transported, as soon as possible, to St. James's Hospital for processing.
Related links
Reference range	Immunofixation (IF): Details the absence (no abnormal bands) or presence of a monoclonal protein. Monoclonal proteins include Free kappa chains (FKC) and Free lambda chains (FLC) (Bence Jones Protein (BJP)) and IgG type Kappa (GK)/IgG type Lambda (GL)/IgA type Kappa (AK)/IgA type Lambda (AL)/IgM type Kappa (MK)/IgM type Lambda (ML).
Last updated	Tue, 09 Jun 2020 15:43:40 IST