Name |
Double Stranded DNA (dsDNA) Antibodies |
Alternative name/Profile |
DNAE, Anti-DNA, Connective Tissue Disease, CTD, Systemic Lupus Erythematosis (SLE), ANA, ENAE, Crithidia, DNAC, DNAT, dsDNA |
Department |
Immunology |
Investigation |
Screen: Immunoassay (Phadia 2500)
Confirmatory: Indirect Immunofluorescence (Aesku nDNA (Crithidia Luciliae) Slides) |
Specimen type |
Clotted blood
|
Sample type |
Serum |
Sample container & volume |
5ml Red. |
Frequency of analysis |
DNAE - Daily, Monday to Friday
DNAC = Batch Basis
|
Turnaround time |
DNAE = 9 days
DNAC = 14 days |
Availability |
|
Notes |
Useful for:
- Investigating patients for Systemic Lupus Erythematosis (SLE).
- Monitoring patients with Systemic Lupus Erythematosis (SLE).
Clinical Information:
- IgG antibodies directed against Double Stranded DNA (dsDNA) are present in up to 70% of patients with Systemic Lupus Erythematosis (SLE) and form part of the diagnostic criteria.
- Levels of Double Stranded DNA (dsDNA) antibodies correlate with disease activity and can increase prior to the start of clinical symptoms.
Please Note:
- All patients that have an equivocal or positive Connective Tissue Disease (CTD) test will have a Double Stranded DNA (dsDNA) test and Extractable Nuclear Antigens (ENA) test performed. This is requested by the laboratory.
- Samples that are negative for Connective Tissue Disease (CTD) test will not have a Double Stranded DNA (dsDNA) test performed.
- All samples positive for Double Stranded DNA (dsDNA) antibodies by immunoassay, will have a Double Stranded DNA (dsDNA) Crithidia antibody assay performed. This is requested by the laboratory.
- Where External Institiutions cannot guarantee immediate delivery, samples must be centrifuged, separated and stored at 4oC. Samples can then be transported, as soon as possible, to St. James's Hospital for processing.
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Related links |
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Reference range |
Immunoassay (Phadia 2500)
<10 IU/ml = Negative
10-15 IU/ml = Equivocal
>15.0 IU/ml = Positive
Reference Ranges provided by Phadia 2014.
Indirect Immunofluorescence (Inova dsDNA (Crithidia Luciliae) Slides)
NEG - Negative
1:20 - Positive at 1:20
1:40 - Positive at 1:40
1:80 - Positive at 1:80
1:160 - Positive at 1:160 |
Last updated |
Fri, 19 Jul 2024 09:36:56 IST |
