Name |
Oxidative Burst by Flow Cytometry (OBFC) |
Alternative name/Profile |
Neutrophil Function, Chronic Granulomatous Disease (CGD), Dihyrorhodamine (DHR) |
Department |
Immunology |
Investigation |
Flow Cytometry (BD FACSCanto II) |
Specimen type |
Blood |
Sample type |
EDTA (Blood) |
Sample container & volume |
4ml Purple EDTA. for patient sample and travel control |
Frequency of analysis |
Daily, Monday to Friday |
Turnaround time |
2 days |
Availability |
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Notes |
Useful for:
- Investigating patients for Chronic Granulomatous Disease (CGD).
Clinical Information:
- Chronic Granulomatous Disease (CGD) is caused by a genetic mutation. This mutation results in an inability of phagocytes to produce the reactive oxygen species (ROS) involved in the oxidative burst responce to inflammatory stimuli.
- Consequently patients with Chronic Granulomatous Disease (CGD) have recurrent life-threatening pyogenic infections that produce widespread graulomatous inflammation in the tissue.
- An inability to produce reactive oxygen species (ROS) can be indirectly detected in the laboratory by assessing the ability of a patient's stimulated neutrophils to oxidise dihyrorhodamine (DHR).
Please Note:
- Myeloperoxidase (MPO) deficieny can also result in reduced neutrophil activity.
- Samples for Oxidative Burst by Flow Cytometry (OBFC) are processed by arrangement with the laboratory. Please ring 01 4162927 to arrange sample processing.
- Samples for Oxidative Burst by Flow Cytometry (OBFC) must be kept at ambient room temperature and sent immediately for processing.
- Please note a travel control must be sent with each sample.
- The sample receipt cut-off time for Oxidative Burst by Flow Cytometry (OBFC) is 13.00 daily.
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Related links |
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Reference range |
Stimulation Index (SI) >100 - Normal
Stimulation Index (SI) =
Median Fluorescence of DHR+stimulated population ⁄ Median Flourescence of DHR+ unstimulated population
This value was obtained by an in-house validation of 20 patients, all with >90% DHR+ cells after stimulation with PMA, in September 2016. |
Last updated |
Fri, 14 Jan 2022 16:04:19 GMT |