Immunology

Full details for "Naive and Memory B-Cells".

Name

Naive and Memory B-Cells

Alternative name/Profile

Class Switched Memory B-Cells (CSMB), Transitional B-Cells, Surface Markers/Receptors, B cells, B cell, Extended Panel, CVID, Flow Cytometry, IgM, Class Switching, Immunodeficiency, Mature, Phenotype, Phenotyping, T-cells, T-cell, T cell, T cells, Subset, Subsets, Immunophenotyping, Humoral, Cyclophilin Ligand, CAML, Cyclophilin Ligand Interactor, TACI, B-cell-activating factor receptor, BAFF-R

Department

Immunology

Investigation

Flow Cytometry (BD FACSCanto II)

Specimen type

Blood

Sample type

EDTA (Blood)

Sample container & volume

4ml Purple EDTA

Frequency of analysis

Daily Monday to Friday

Turnaround time

4 days

Availability

Notes

Useful for:

Screening patients for Common Variable Immunodeficiency (CVID)
Screening patients for Hyper-IgM Syndrome
Assessing B-Cell subset reconstitution post bone marrow/stem cell transplant
Assessing B-Cell subset responce to B-Cell depleting immunotherapy

Clinical Information:

This assay provies quantitative B-Cell values in respect to the total number of peripheral B-Cells.
Common variable immunodeficiency (CVID), a disorder of B-cell function, is characterized by hypogammaglobulinemia. In CVID different B-cell subsets are associated with particular clinical phenotypes or presentations.
Hyper-IgM syndromes are characterized by increased or normal leveles of IgM with low IgG. Patients with hyper-IgM syndromes have a defect in isotype class-switching, which leads to a decrease in Class-Switched Memory B-cells (CSMB), with or without an increase in Non-Switched Memory B-cells and IgM-only Memory B-cells.

Please Note:

As this is a specialised test please contact the laboratory to arrange sample processing.
Absolute lymphocyte levels are influenced by circadian variation, therefore consistency of timing when acquiring blood samples is required when monitoring patients serially.
Samples must be kept at ambient room temperature and analysed within 24 hours of sample collection.
The cut-off time for receipt of samples is approximatly 15.00 (12.00 on Fridays).

Related links

Reference range

	Naive B-Cell (CD27-, IgD+)		Memory B-Cell (CD27+, IgD+)
Age (Years)	Percent (%)	Count (10^6/L)	Percent (%)	Count (10^6/L)
0-1	91-96	537-1179	2-4	12-36
2-3	83-90	571-1323	4-7	38-90
4-5	76-85	334-611	4-9	25-60
6-10	70-80	133-389	7-12	22-43
11-18	75-87	171-293	5-10	12-32
Adult*	51-88	83-168	1.8-17.4	0-41

Reference ranges for children aged 0 months to 18 years adapted from 'Reference Values for B cell Subpopulations from Infancy to Adulthood', H. Morbach et al. 2010, Clinical and Experimental Immunology, 162: 271-279.

Adult* reference range established in the Immunology Department, St James's Hospital, 2015, on 10 healthy normal controls (5-95% percentile).

Last updated

Fri, 23 Sep 2016 16:09:04 IST