Name |
B-Cell and Natural Killer (NK) Cell Quantitiation by Flow Cytometry |
Alternative name/Profile |
Lymphocyte subsets, B-Cell Subsets, B-Cells, CD19 Count, CD16 Count, CD56 Count, NK cells, Immunodeficiency, Immunophenotyping, flow cytometry, B Cell, B Cells, TBNK, LSS |
Department |
Immunology |
Investigation |
Flow Cytometry (BD FACSCanto II) |
Specimen type |
Blood |
Sample type |
EDTA (Blood) |
Sample container & volume |
4ml Purple EDTA |
Frequency of analysis |
Daily Monday to Friday |
Turnaround time |
2 days |
Availability |
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Notes |
Useful for:
- Investigating patients with Primary Immunodeficiency.
- Immune monitoring in patients recieving immunosupressive therapy.
- Assessment of immune reconstitution post hematopoietic cell transplantation.
- Investigation of anomalies in lymphocyte subsets due to infection or malignancy.
- Absolute quantitation of circulating B-Cells in Chronic Lymphocytic Leukemia.
Clinical Information:
- Lymphocytes can be generally classified into T-, B- and Natural Killer (NK-) cells which can be distinguished, by flow cytometry, using a combination of one or more cell surface markers.
- B-Cells are detected using the surface marker CD19.
- Natural Killer (NK) cells are detected using the surface markers CD16 and CD56.
- Biological factors such as temperature, hormones, circadian variation and the environment can influence absolute lymphocyte levels. It is accepted that, in general, B-Cell levels increase between morning and noon and that Natural Killer (NK) cells remain constant.
Please Note:
- Where External Institiutions cannot guarantee immediate delivery, samples must be kept at room temperature until they are transported to St. James's Hospital for processing.
- As absolute lymphocyte levels are influenced by circadian variation, consistency of timing when aquiring blood samples is required when monitoring patients serially.
- The request 'Lymphocyte Subsets' only provides T-Cell quantitation. If B-Cell and Natural Killer (NK) cell quantitiation is required the request 'TBNK' should be used, or this must be specified on the form.
- Samples must be kept at ambient room temperature and analysed within 24 hours of sample collection.
- The cut-off time for receipt of samples is approximatly 15.00.
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Related links |
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Reference range |
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B-Cells (CD19)
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Natural Killer (NK) Cells (CD16 and CD56)
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Age
|
Percentage (%)
|
Absolute Count (x10^6/L)
|
Percentage (%)
|
Absolute Count (x10^6/L)
|
|
0-3 Months
|
6-32
|
300-2000
|
4-18
|
170-1100
|
|
3-6 Months
|
11-41
|
430-3000
|
3-14
|
170-830
|
|
6-12 Months
|
14-37
|
610-2600
|
3-15
|
160-950
|
|
1-2 Years
|
16-35
|
720-2600
|
3-15
|
180-920
|
|
2-6 Years
|
14-33
|
370-1400
|
4-17
|
130-720
|
|
6-12 Years
|
13-27
|
270-860
|
4-17
|
100-480
|
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12-18 Years
|
6-23
|
110-570
|
3-22
|
70-480
|
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Adult
|
5-19
|
99-618
|
4-24
|
72-577
|
Adult Reference Ranges validated in-house 2012.
Paediatric Ranges adopted from Shearer WT, Rosenblatt HM, Gelman RS et al. Lymphocyte subsets in 817 healthy children from birth through 18 years of age: The Paediatric AIDS Clinical Trials Group P1009 study, J. Allergy Clinical Immunology 2003; 112(5): 973-980.
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Last updated |
Fri, 10 Apr 2020 08:49:58 IST |
