Name |
Class-Switched Memory B cells (CSMB) and CD21 Low B-Cells |
Alternative name/Profile |
Naive and Memory B-Cells, Transitional B-Cells, Surface Markers/Receptors, B cells, B cell, Extended Panel, CVID, Flow Cytometry, IgM, Class Switching, Immunodeficiency, Mature, Phenotype, Phenotyping, T-cells, T-cell, T cell, T cells, Subset, Subsets, Immunophenotyping, Humoral, Cyclophilin Ligand, CAML, Cyclophilin Ligand Interactor, TACI, B-cell-activating factor receptor, BAFF-R |
Department |
Immunology |
Investigation |
Flow Cytometry (BD FACSCanto II) |
Specimen type |
Blood |
Sample type |
EDTA (Blood) |
Sample container & volume |
4ml Purple EDTA |
Frequency of analysis |
Daily Monday to Friday |
Turnaround time |
4 days |
Availability |
|
Notes |
Useful for:
- Screening patients for Common Variable Immunodeficiency (CVID)
- Screening patients for Hyper-IgM Syndrome
- Assessing B-Cell subset reconstitution post bone marrow/stem cell transplant
- Assessing B-Cell subset responce to B-Cell depleting immunotherapy
Clinical Information:
- This assay provies quantitative B-Cell values in respect to the total number of peripheral B-Cells.
- Common variable immunodeficiency (CVID), a disorder of B-cell function, is characterized by hypogammaglobulinemia. In CVID different B-cell subsets are associated with particular clinical phenotypes or presentations.
- Hyper-IgM syndromes are characterized by increased or normal leveles of IgM with low IgG. Patients with hyper-IgM syndromes have a defect in isotype class-switching, which leads to a decrease in Class-Switched Memory B cells (CSMB), with or without an increase in Non-Switched Memory B-cells and IgM-only Memory B-cells.
- An increase in CD21 low IgD+ B-Cells has been demonstrated in some Common Variable Immunodeficiency (CVID) patients.
Please Note:
- As this is a specialised test please contact the laboratory to arrange sample processing.
- Absolute lymphocyte levels are influenced by circadian variation, therefore consistency of timing when acquiring blood samples is required when monitoring patients serially.
- Samples must be kept at ambient room temperature and analysed within 24 hours of sample collection.
- The cut-off time for receipt of samples is approximatly 15.00 (12.00 on Fridays).
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Related links |
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Reference range |
| |
Class-Switched Memory B-Cells (CSMB)
(CD27+, IgD-)
|
CD21 Low B-Cells |
| Age (Years) |
Percent (%) |
Count (10^6/L) |
Percent (%) |
Count (10^6/L) |
| 0-1 |
0.1-2 |
1-16 |
0.3-4 |
3-35 |
| 2-3 |
1-4 |
13-42 |
1.8-3.6 |
15-36 |
| 4-5 |
3-7 |
16-44 |
1.8-5.2 |
8-23 |
| 6-10 |
5-12 |
16-31 |
0.9-3.5 |
3-4 |
| 11-18 |
3-10 |
10-29 |
0.9-3.3 |
2-12 |
| Adult* |
4-22 |
2-49 |
0.8-3.2 |
2-5 |
Reference ranges for children aged 0 months to 18 years adapted from 'Reference Values for B cell Subpopulations from Infancy to Adulthood', H. Morbach et al. 2010, Clinical and Experimental Immunology, 162: 271-279.
Adult* reference range established in the Immunology Department, St James's Hospital, 2015, on 10 healthy normal controls (5-95% percentile). |
Last updated |
Thu, 22 Aug 2019 11:44:48 IST |
