Name |
Liver/Kidney Microsome Antibodies (LKM) |
Alternative name/Profile |
Microsomal antibodies, Mitochondrial antibodies (MITA), Smooth Muscle Antibodies (SMA), Pareital Cell Antibodies (PCA), Antibody Screen (AS), Type-2 Autoimmune Hepatitis, Cytochrome Monooxygenase P450, blot |
Department |
Immunology |
Investigation |
Indirect Immunofluorescence (Rat, Liver, Kidney, Stomach Substrate Slides) and Immunoblot (EUROLINE Autoimmune Liver Diseases (IgG)) |
Specimen type |
Clotted blood
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Sample type |
Serum |
Sample container & volume |
5ml Red |
Frequency of analysis |
Indirect Immunofluorescence Daily Monday to Friday and follow on Liver Immunoblot on a batch basis. |
Turnaround time |
6 days for Indirect Immunofluorescence and 21 days for Liver Immunoblot.
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Availability |
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Notes |
Useful for:
- Investigating patients suspected of having Autoimmune Hepatitis.
Clinical Information:
- Autoimmune liver diseases are characterised by the presence of highly specific antibodies against nuclear, mitochondrial, microsomal, and cytoplasmic antigens.
- Liver/Kidney Microsome (LKM) Antibodies are a serologic marker for Type-2 Autoimmune Hepatitis - a subtype of Autoimmune Hepatitis.
- Liver/Kidney Microsome (LKM) Antibodies are generally directed against the enzyme Cytochrome Monooxygenase P450.
- Patients with Type-2 Autoimmune Hepatitis tend to be young females with a severe disease that responds well to immunosupressive therapy.
Please Note:
- Some patients with Hepatitis C infections can also have Liver/Kidney Microsome (LKM) Antibodies.
- All samples that are positive for Liver/Kidney Microsome (LKM) Antibodies are titred at 1:40, 1:80 and 1:160. This is requested by the laboratory.
- Samples with unusual staining in the liver will have an immunoblot performed. This is requested by the laboratory.
- The liver immunoblot includes the antigens AMA-M2, M2-3E (BPO), Sp100, PML, gp210, LKM-1, LC-1, SLA/LP and Ro-52.
- As the substrate (Nova Lite Rat, Liver, Kidney, Stomach Substrate Slides) in this assay is also used for the detection of Smooth Muscle Antibodies, Parietal Cell Antibodies and Mitochondrial Antibodies, all requests for Liver/Kidney Microsome (LKM) Antibodies also have Smooth Muscle Antibodies, Parietal Cell Antibodies and Mitochondrial Antibodies requested.
- Where External Institiutions cannot guarantee immediate delivery, samples must be centrifuged, separated and stored at 4oC. Samples can then be transported, as soon as possible, to St. James's Hospital for processing.
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Related links |
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Reference range |
Immunofluorescence-
Negative
Positive at a titre value of 1:40
Positive at a titre value of 1:80
Positive at a titre value of 1:160
Liver Immunoblot
Negative
Weak Positive
Positive
Strong Positive
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Last updated |
Fri, 19 Jul 2024 09:36:56 IST |
