Name |
Naive and Memory B-Cells |
Alternative name/Profile |
Class Switched Memory B-Cells (CSMB), Transitional B-Cells, Surface Markers/Receptors, B cells, B cell, Extended Panel, CVID, Flow Cytometry, IgM, Class Switching, Immunodeficiency, Mature, Phenotype, Phenotyping, T-cells, T-cell, T cell, T cells, Subset, Subsets, Immunophenotyping, Humoral, Cyclophilin Ligand, CAML, Cyclophilin Ligand Interactor, TACI, B-cell-activating factor receptor, BAFF-R |
Department |
Immunology |
Investigation |
Performed in house by Flow Cytometry (BD FACSCanto II) |
Specimen type |
Blood |
Sample type |
EDTA (Blood) |
Sample container & volume |
4ml Purple EDTA |
Frequency of analysis |
Daily Monday to Friday |
Turnaround time |
4 days |
Availability |
Available to SJH and External Institutions. Not available to GPs. |
Notes |
Useful for:
- Screening patients for Common Variable Immunodeficiency (CVID)
- Screening patients for Hyper-IgM Syndrome
- Assessing B-Cell subset reconstitution post bone marrow/stem cell transplant
- Assessing B-Cell subset response to B-Cell depleting immunotherapy
Clinical Information:
- This assay provides quantitative B-Cell values in respect to the total number of peripheral B-Cells.
- Common variable immunodeficiency (CVID), a disorder of B-cell function, is characterized by hypogammaglobulinemia. In CVID different B-cell subsets are associated with particular clinical phenotypes or presentations.
- Hyper-IgM syndromes are characterized by increased or normal levels of IgM with low IgG. Patients with hyper-IgM syndromes have a defect in isotype class-switching, which leads to a decrease in Class-Switched Memory B-cells (CSMB), with or without an increase in Non-Switched Memory B-cells and IgM-only Memory B-cells.
Please Note:
- External Institutions must send a recent FBC result with patient sample.
- Absolute lymphocyte levels are influenced by circadian variation, therefore consistency of timing when acquiring blood samples is required when monitoring patients serially.
- Samples must be kept at ambient room temperature and analysed within 24 hours of sample collection.
- The cut-off time for receipt of samples is approximately 15.00 (12.00 on Fridays).
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Related links |
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Reference range |
|
|
Naive B-Cell (CD27-, IgD+)
|
Memory B-Cell (CD27+, IgD+) |
| Age (Years) |
Percent (%) |
Count (10^6/L) |
Percent (%) |
Count (10^6/L) |
| 0-1 |
91-96 |
537-1179 |
2-4 |
12-36 |
| 2-3 |
83-90 |
571-1323 |
4-7 |
38-90 |
| 4-5 |
76-85 |
334-611 |
4-9 |
25-60 |
| 6-10 |
70-80 |
133-389 |
7-12 |
22-43 |
| 11-18 |
75-87 |
171-293 |
5-10 |
12-32 |
| Adult* |
51-88 |
83-168 |
1.8-17.4 |
0-41 |
Reference ranges for children aged 0 months to 18 years adapted from 'Reference Values for B cell Subpopulations from Infancy to Adulthood', H. Morbach et al. 2010, Clinical and Experimental Immunology, 162: 271-279.
Adult* reference range established in the Immunology Department, St James's Hospital, 2015, on 10 healthy normal controls (5-95% percentile). |
Last updated |
Fri, 05 Jun 2026 14:27:56 IST |
