Histopathology

Name	Specimen type	Sample container & volume	Frequency of analysis
Bile Duct Stent, Bile Duct Brushing(Cytology)	Fixed in Cytolyt	Fixative- Cytolyt. Available in diagnostic imaging.	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Bone Marrow Biopsy	Fixed in 10% Formalin	10% Formalin - Pre-filled Pot	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Bronchial brush (Cytology)	Cytolyte fixative	Cytolyte fixative available in diagnostic imaging	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Bronchial Wash(Cytology), Lavage, aspirate, bronchoalveolar lavage.	Fresh	Sterile specimen container (between 30ml and 70ml) Contact Materials Management 416 2573/416 2574	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Cilia	Ciliary brushings	3% Glutaraldehyde. Contact Histology laboratory at 4162033 for these bottles	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
CSF for Cytology	Fresh	30 mL universal. Contact Materials Management 416 2573/ 416 2574	Must be received in Cytology within 24hrs of collection.

Name	Specimen type	Sample container & volume	Frequency of analysis
Dental Specimens	Fixed in 10% Buffer Formalin	Pre filled pots, Contact Histopathology 4162033 for a list of suppliers	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Endoscopic Biopsies	Fixed in 10% Buffer Formalin	Pre-filled pot contact Pharmacy ext 2553	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Ex-vivo (cytology)	Fresh tissue sample. Unfixed lymph node measuring approx 1cm x 0.5cm x 0.5cm in RPMI. NB: Please do not send core biopsies	Unfixed tissue in a universal container containing 10ml of RPMI	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Flow Cytometry Requests	Receive fresh in RPMI medium	30 ml universal Contact Cytology laboratory 416 2905	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Fluids for Cytology(pleural, peritoneal, pericardial, cyst contents, bile etc)	Receive fresh, representative samples	Sterile specimen container (sized between 30 and 70 mL) please no bags or buckets Contact Materials Management ext 4162573/4162574 for Universal containers	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
FNA(Fine Needle Aspirates)for Cytology	Fixed - Cytolyte	Pre-filled 30 mL universal available in diagnostic imaging.	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
FNA air dried prepared slides	Unfixed	Labelled slide in plastic slide container . Contact Cytopathology Lab 416 2905	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Frozen sections	Fresh unfixed	Pot or bucket Contact Materials Management Tel 4162574	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Histology specimens from GPs and external institutions	Fixed in 10 % Bufferd Formalin	Pre filled pots contact Histopathology 4162033 for a list of suppliers	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Histopathology & Culture	Fresh for microbiology. Fixed for Histopathology	N/A	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Large Head/Neck Specimens	10% Buffer Formalin	Boxes/Pins/Cork Contact Histopathology ext 2995 for boxes etc. Contact pharmacy ext 2553 for formalin	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Large Resection Specimens	10% Buffer Formalin Contact pharmacy ext 2553	Buckets appropriate for the size of specimen. Contact materials management ext 2574	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Limbs	Fixed in 10% buffered Formalin	N/A	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Lymphoma Studies	Fresh unfixed tissue.	N/A	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
MOHs	Fresh Unfixed	Pot or bucket contact Materials Management Tel 4162574	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Muscle Biopsy	Fresh	Wrap fresh in lightly moistened saline gauze and transport as per frozen sections .	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Oesophageal Brushings (Cytology)	Fixed in Cytolyte	Pre-filled 30 mL universal. Contact Cytopathology laboratory 416 2905	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Peripheral Nerve	Fresh	see Notes	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Flourescent In Situ Hybridisation	Unstained slides/Paraffin Block	N/A	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Immunohistochemistry	Unstained slides/Paraffin Block	N/A	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Renal Biopsy	Fresh in saline	N/A	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Respiratory Bronchial Brush(Cytology)	Fixed in Cytolyte	Pre-filled 30 mL universal, available in diagnostic imaging.	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Samples from patients at risk of TSE	Fixed in 10% buffer formalin	Pre filled pot in plastic bag placed in rigid Biohazard container. Contact Materials Management ext 4162573/4162574	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Sentinel Nodes	Fresh	N/A	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Skin/Small Surgical Specimens	10% Buffer Formalin	Pre-filled pot Contact Pharmacy ext 4162553	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Soft tissue sarcoma	Fresh	Materials management ext 4162573/4162574	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Sputum (Cytology)	Fresh	Sterile specimen container between 30ml and 70ml. Contact materials management 416 2573/416 2574	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Synovial Fluid (crystals)	Fresh. MUST be received in Cytology within 1 hour.	5-20 mL in 30 mL universal Materials Management 416 2573/ 416 2574	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Unusual Tumours/Disease	Please discuss with Histopathology department ex 2033	N/A	N/A

Name	Specimen type	Sample container & volume	Frequency of analysis
Urine (Cytology)	Receive fresh	Sterile specimen container (sized between 30 and 70 mL) Materials Management 416 2573/416 2574	N/A

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